Comparative study of the influence of colloid-hyperosmolar and protein-salt solutions on anti-inflammatory and detoxification processes in rats with burn shock
Objective. Experimentally justify the use of colloidal-hyperosmolar solution based on hydroxyethyl starch HAES-LX-5 % and protein-salt solution based on albumin Lactoprotein with sorbitol (LPS) in burn shock (BS).
Materials and methods. The experiments were performed on an experimental model of OS in white male rats. HAES-LX-5 %, LPS solution and control 0.9 % NaCl solution at a dose of 10 ml/kg/day for 7 days were used for infusion therapy. The blood leukocyte intoxication index (LII), medium weight molecules (MWM), C-reactive protein (CRP) were studied.
Results and discussion. In the conditions of experimental BS with the introduction of 0.9 % NaCl, the LII on the 7th day significantly increased by 5.37 times (p<0.01) compared with intact animals (0.887±0.108 and 0.165±0.098, respectively). This indicates the development of destructive processes and intoxication of rats in BS. When HAES-LX-5 % and LPS were administered, LII increased only 2.33 times (p<0.01) and 2.41 times (p <0.05) and was significantly lower compared to 0.9 % NaCl. In animals from the group of control pathology there was an increase in blood levels of MWM, on the 1st day by 75.6 %, on the 3rd day – by 159.5 %, and on the 7th day – by 128.0 %, respectively, compared with intact animals. A systemic inflammatory reaction was also formed, which was manifested by an increase in the level of CRP. Pharmacological correction of BS with HAES-LX-5 % and LPS solutions equally inhibited the development of endotoxicosis from the 3rd to the 7th day, which was accompanied by significant (p<0.05) decrease in the level of MWM by 36.2 % and 42.5 %, respectively, after administration of HAES-LX-5 % and by 32.9 % and 38.8 % after administration of LPS relative to control pathology. The use of solutions of HAES-LX-5 % and LPS inhibited the development of inflammation from the 3rd to the 7th day, which was accompanied by a significant (p<0.05) decrease in the level of CRP by 30.7 % and 35.0 % for HAES-LX-5 %, by 29.4 % and 32.9 % for LPS compared to control.
Conclusions. It has been experimentally shown in the model of BS that solutions HAES-LX-5 % and LPS show anti-inflammatory activity and reduce the level of endogenous intoxication, inhibiting the development of systemic stress.
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