Fluorescent in situ hybridization in patients with chronic lymphocytic leukemia with autoimmune hemolytic anemia

  • M.O. Valchuk State Institution “Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine”, Lviv, Ukraine
  • O.V. Zotova State Institution “Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine”, Lviv, Ukraine
  • A.S. Lukyanova Medical and Biological Center “Genom”, Kyiv, Ukraine
  • O.Ya. Vyhovska State Institution “Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine”, Lviv, Ukraine
  • Yu.S. Karo Municipal Non-Commercial Enterprise “5th City Clinical Hospital”, Lviv, Ukraine
  • L.M. Lukavetsky State Institution “Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine”, Lviv, Ukraine
  • Ya.I. Vyhovska State Institution “Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine”, Lviv, Ukraine
  • V.E. Logininsky State Institution “Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine”, Lviv, Ukraine
  • O.O. Shalay State Institution “Institute of Blood Pathology and Transfusion Medicine of the National Academy of Medical Sciences of Ukraine”, Lviv, Ukraine
DOI: https://doi.org/10.32902/2663-0338-2020-3.1-12

Abstract

Background. Gene aberrations are an important prognostic criterion for the course of B-cell chronic lymphocytic leukemia (B-CLL) and response to treatment, which includes not only immunochemotherapy, but also concomitant infusion therapy for the prevention and correction of complications.

Objective. To investigate the presence of prognostic cytogenetic changes in patients with B-CLL with autoimmune hemolytic anemia (AIGA). To analyze the course of the disease and the direct effect of treatment in patients with cytogenetic changes of different nature.

Materials and methods. Cytogenetic studies were performed by fluorescent in situ hybridization (FISH) on the interphase nuclei of peripheral blood lymphocytes in 11 patients with B-CLL with AIGA. Probes to the ATM genes (gene localized in region 11q23) and TP53 (gene localized in region 17p13) were used in the work, the deletions of which have prognostic value in B-CLL. All patients received treatment.

Results. Among 11 patients with AIGA, signals to both genes were detected in nuclei 4. No deletions were detected. In the cells of the other 7 patients, the absence of a single signal to the ATM gene was detected, indicating the presence of a deletion of del(11)(q23). In recent patients, an unfavorable course of B-CLL disease was observed without response to treatment. Deletions of the TP53 gene in patients of the studied group were not detected.

Conclusions. FISH study in patients with B-CLL with AIGA revealed the presence of important and prognostically unfavorable chromosomal rearrangement of the ATM gene in 63 % of patients.

Keywords: B-cell lymphocytic leukemia, cytogenetic FISH study, ATM gene, TP53 gene.
Published
2020-10-21
How to Cite
Valchuk, M., Zotova, O., Lukyanova, A., Vyhovska, O., Karo, Y., Lukavetsky, L., Vyhovska, Y., Logininsky, V., & Shalay, O. (2020). Fluorescent in situ hybridization in patients with chronic lymphocytic leukemia with autoimmune hemolytic anemia. Infusion & Chemotherapy, (3.1), 16-16. https://doi.org/10.32902/2663-0338-2020-3.1-12
Issue
No. 3.1 (2020): INFUSION & CHEMOTHERAPY
Section
Materials of IV International Congress of infusion therapy
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This work is licensed under a Creative Commons Attribution 4.0 International License.

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